toxic effects of hydroalcoholic extract of kiwi (actinidia chinensis) on histological structure of the male sprague-dawley rat reproductive tissues

hormonally active agents are widespread in the environment and exist in the human diet. there isinsufficient knowledge regarding the influence of phytochemicals on the pathophysiology of the malereproductive system. kiwi is a rich source of phytochemicals such as flavones (with estrogenic activity),serotonin and vitamin c. these components can influence the hormones and interfere with reproductiveactivities. therefore, the objective of this project was to determine whether extract of kiwi could change thestructure of male reproductive tissues. so, 40 male rats were divided into four groups. three experimentalgroups were fed with 75, 100 and 150 mg/kg of kiwi extract and control groups with solvent for 50 days.then, rats were sacrificed and their testis, ductus deferens, seminal vesicle, prostate and epididymidis wereremoved, fixed, processed and stained with h & e and acridine orange. the specimens were studied underlight and fluorescent microscopes. histological observations revealed some changes in the structure of testes,but not in the other parts of male reproductive tissues. some spermatocytes have become fusiform and thenumber of these cells has been increased, dose dependently. there were many spermatocyes at the metaphasestage, among 100 and 150 mg/kg treated groups; some of the mitotic figures, sperm, most of thespermatogonia and spermatocytes stained red with acridine orange, which indicats denaturation of dnastrands. a few fragmented nuclei were also observed in the 150mg/kg treated group. in conclusion, it seemsthat extract of kiwi can change the spermatocyte cytoarchitecture and affects the spermatogonia andspermatozoa. it may exert dual effects on the proliferating cells, such as spermatogenesis linage. in lowconcentrations it could induce proliferation, and in high concentrations, it may lead to cell death and nucleusfragmentation. the extract may elongate the period of the metaphase.